PI susceptibilities of pre-PI and failure virus from two patients demonstrate changes in phenotypic susceptibility over time.

<p>Full-length Gag-Protease sequences from pre-PI therapy and failure time-points was amplified or synthesised, and single round phenotypic susceptibility testing performed. PIs tested were Lopinavir (LPV) and Darunavir (DRV). (A) Susceptibility data from patient 5 demonstrates the reduction in susceptibility conferred by major mutations I84V (black) and I54V (grey) in our system in comparison with the patient virus at pre-PI therapy (white). Error bars represent standard deviation of two independent experimental repeats. (B) Virus derived pre-PI therapy (white bar) and at time of failure (black bar) from patient 6 demonstrated a difference in susceptibility to LPV in the absence of the development of major resistance mutations.