Additional file 12: of Transcriptome analysis of 20 taxonomically related benzylisoquinoline alkaloid-producing plants

Phylogenetic analysis of 2-oxoglutarate/iron (II)-dependent dioxygenase (DIOX) gene candidates from twenty BIA-accumulating plant species. Red text denotes characterized genes or enzymes used as tBLASTn queries for transcriptome mining. Black text denotes uncharacterized gene candidates identified through mining (>40 % identity to queries). Bootstrap values for each clade were based on 1000 iterations. Each candidate is labeled with respective species abbreviation (e.g. AME, Argemone mexicana; see Table 1) and candidate number (e.g. DIOX1). The queries are derived from Papaver somniferum (PSO). The outgroup is anthocyanidin synthase from Arabidopsis thaliana (labeled ATHDIOX). Amino acid sequences for candidates, queries, and outgroups are found in Additional file 6. (PDF 20162 kb