Additional file 10: of Transcriptome analysis of 20 taxonomically related benzylisoquinoline alkaloid-producing plants

Phylogenetic analysis of berberine bridge enzyme (BBE) gene candidates from twenty BIA-accumulating plant species. Red text denotes characterized genes or enzymes used as tBLASTn queries for transcriptome mining. Black text denotes uncharacterized gene candidates identified through mining (>40 % identity to queries). Species for which tBLASTn querying did not yield hits are not represented on the tree. Bootstrap values for each clade were based on 1000 iterations. Each candidate is labeled with respective species abbreviation (e.g. AME, Argemone mexicana; see Table 1) and candidate number (e.g. BBE1) where more than one hit was identified. Each query is labeled according to species (additional species: PSO, Papaver somniferum; BST, Berberis stolonifera). The outgroup is tetrahydrocannabinolic acid synthase (THCAS) from Cannabis sativa (CSA). Amino acid sequences for candidates, queries, and outgroups are found in Additional file 6. (PDF 1851 kb