Additional file 19: of Transcriptome analysis of 20 taxonomically related benzylisoquinoline alkaloid-producing plants

Phylogenetic analysis of BAHD-type O -acetyltransferase (OAT; D'Aura 2006) gene candidates from twenty BIA-accumulating plant species. Red text denotes the characterized AT1 (1,13-dihydroxy-N-methylcanadine O-acetyltransferase; [6]) and SAT (salutaridine synthase) from Papaver somniferum (PSO) used as tBLASTn queries for transcriptome mining. Black text denotes uncharacterized gene candidates identified through mining (>30 % identity to query). Bootstrap values for each clade were based on 1000 iterations. Each candidate is labeled with respective species abbreviation (e.g. AME, Argemone mexicana; see Table 1) and candidate number (e.g. AT1). The outgroup is vinorine synthase from Rauvolfia serpentina (RSEVS). Amino acid sequences for candidates, queries, and outgroups are found in Additional file 6. (PDF 5574 kb