<i>Spink5</i>^<i>-/-</i><i>Klk5</i>^<i>-/-</i> skin does not show evidence of abnormal epidermal differentiation.

<p>(<b>A, B</b>) IHF analyses show normalized Involucrin and Loricrin expression in <i>Spink5</i>^<i>-/-</i><i>Klk5</i>^<i>-/-</i> skin compared to <i>Spink5</i>^<i>-/-</i><u>as indicated by arrows; scale bar: 25μm</u> (<b>C</b>) Deletion of Klk5 blocks Flg degradation. Immunostaining of Flg extends to the granular layer and the <i>stratum corneum</i> in wt, <i>Klk5</i>^<i>-/-</i>, <i>Spink5</i>^<i>-/-</i><i>Klk5</i>^<i>-/-</i> while it is drastically reduced in the granular layer and restricted to the detached <i>stratum corneum</i> in <i>Spink5</i>^<i>-/-</i> mice <u>as indicated by arrows; scale bar: 25μm.</u>