Additional file 1: of Small RNAs from the wheat stripe rust fungus (Puccinia striiformis f.sp. tritici)

Bioinformatics Pipeline. Graphical summary of the bioinformatic pipeline used to obtain putative Puccinia striiformis small RNAs (Pst-sRNAs). The total sRNA library consists of mostly wheat reads (green) with a small fraction of stripe rust reads. After mapping to the stripe rust genome, discarding reads present in uninfected controls, and discarding sequences matching wheat miRNA and protein-coding sequences, the library is increasingly enriched for stripe rust reads (orange). Sequences discarded at each step are shown as arrows to the right. (PDF 36 kb