The yeast Snf1 pathway is involved in ER stress response.

<p>(A) ER stress resistance caused by deletion of the <i>snf1</i> gene. Wild-type (WT) and <i>snf1</i>Δ mutant strains harboring the indicated multicopy plasmids were spotted onto SD medium lacking or containing 1.5 μg/ml tunicamycin (TM) and incubated at 25°C. Wild-type Snf1 and Snf1(K84M) proteins were expressed in comparable amounts as shown in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005491#pgen.1005491.s005" target="_blank">S3C Fig</a>. (B) ER stress sensitivity caused by expression of the hyperactive form of Snf1. <i>snf1</i>Δ mutant strains harboring the indicated multicopy plasmids were spotted onto SD medium lacking or containing 1 μg/ml tunicamycin (TM) and incubated at 25°C. Wild-type Snf1 and Snf1(G53R) proteins were expressed in comparable amounts as shown in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005491#pgen.1005491.s005" target="_blank">S3D Fig</a>. (C) ER stress sensitivity caused by deletion of the <i>snf4</i> gene encoding the gamma subunit of the Snf1 complex. Wild-type (WT) and <i>snf4</i>Δ, <i>snf1</i>Δ, and <i>snf4</i>Δ <i>snf1</i>Δ mutant strains were spotted onto YPD medium lacking or containing 2 μg/ml tunicamycin (TM) and incubated at 25°C. (D) ER stress resistance caused by deletion of the genes encoding the beta subunits of the Snf1 complex. Wild-type (WT) and <i>sip1</i>Δ, <i>sip2</i>Δ, <i>gal83</i>Δ, and <i>sip1</i>Δ <i>sip2</i>Δ <i>gal83</i>Δ mutant strains were spotted onto YPD medium lacking or containing 1.5 μg/ml tunicamycin (TM) and incubated at 25°C.