Structures and genotyping of <i>Bmal1</i>^<i>GTΔC</i> mice.

<p>(A) The genomic structure of ES cells harboring <i>Bmal1</i>^<i>GTΔC</i> allele. Black and grey colors indicate the exon (E) and intron (I) region, respectively. Above panel represents intact organization of Bmal1 DNA and below panel show the insertion site of the gene-trap vector. Black triangle indicates the location of the primer sequence. (B) Domains of WT and BMAL1^GTΔC proteins. (C) The genotypes were determined by using three primers PCR method (Bmal1^wt-F, Bmal1^WT-R and Bmal1^GTΔC-R). (D) The genotypes were also determined by western blotting of β-GAL.