Identification of alpha and gamma motoneurons that innervate cranial muscles.

<p><b>(a)</b> Rat brainstem section containing trigeminal and lateral facial motor nuclei. The section is labeled with anti-ChAT (green) anti-NeuN (red) and DAPI (blue). <b>(b)</b> Sample outlines of motoneurons in the trigeminal and lateral facial motor nuclei based on anti-ChAT labeling. <b>(c)</b> anti-ChAT labeling of motoneurons in the trigeminal motor nucleus. <b>(d)</b> anti-NeuN labeling of the neurons in <b>panel c</b>. <b>(e)</b> anti-ChAT and anti-NeuN labeling of the neurons in <b>panel c. (f)</b> anti-ChAT labeling of motoneurons in the facial motor nucleus. <b>(g)</b> anti-NeuN labeling of the neurons in <b>panel f</b>. <b>(h)</b> anti-ChAT and anti-NeuN labeling of the neurons in <b>panel f. (i)</b> Luminance of anti-NeuN label versus area of anti-ChAT label for trigeminal motoneurons in three rats. <b>(j)</b> Luminance of anti-NeuN label versus area of anti-ChAT label for facial motoneurons in three rats. <b>(k)</b> Histograms of the first principal component of log(Luminance) and log(Area) for the trigeminal motoneurons in <b>panel i. (l)</b> Histograms of the first principal component of log(Luminance) and log(Area) for the facial motoneurons in <b>panel j.</b> The raw data for panels i and j are in supplemental information <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1002253#pbio.1002253.s002" target="_blank">S2 Data</a> and that for panels k and l are in supplemental information <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1002253#pbio.1002253.s003" target="_blank">S3 Data</a>.