Gene expression analyses of the CBFs and the CBF regulon in the <i>tcf1-1</i> and wild-type plants.

<p><b>(A)</b> to <b>(C)</b> The levels of <i>CBF1</i>, <i>CBF2</i> and CBF3 transcripts, respectively, in <i>tcf1-1</i> and wild-type plants. The plants were treated with cold (4°C) at the indicated time points. <b>(D)</b> The level of <i>TCF1</i> transcripts in the loss-of-function <i>cbf2</i> mutant plants grown under normal conditions or given a 4°C treatment for 7 day. Transcript levels of the <i>ACT2</i> gene were used as a loading control. <b>(E)</b> to <b>(G)</b> Transcript levels of <i>COR47</i><b>(E)</b>, <i>COR15A</i><b>(F)</b> and <i>RD29A</i><b>(G)</b> in <i>tcf1-1</i> and wild-type plants. The plants were treated with cold (4°C) for the indicated times. <b>(H)</b> Western blot analysis with anti-acetylated H4 antibody (<i>Top</i>) revealed that <i>tcf1-1</i> and WT plants have similar levels of acetylated H4. Histone H4 antibody for core histone H4 was used as the immunoblot control (<i>Bottom</i>).