Overview of amino acid sequence variability among NTS-ID2a fragments of VAR2CSA.

<p>Shannon entropy values calculated on the multiple sequence alignment of 90 NTS-ID2a amino acid sequences transcribed by parasites collected from pregnant women, were computed. VAR2CSA domains structure and regions covered by sequencing (NTS: N-terminal segment; DBL, Duffy-binding like; ID: inter-domain region) are indicated. Dotted horizontal lines indicate median of all entropy values (H = 0.39). Black-colored boxes (under graphs) delimit conserved regions (CR1-6), while dotted lines indicate variable regions (VR1-6).