Schematic presentation of the experimental protocols used in the long term diet studies.

<p>From 12 weeks of age female <i>Trpm5</i>^<i>-/-</i> and wild type mice were fed normal mouse chow together with a cafeteria diet (high fat-high sugar) (n = 5-7/strain) (A) or a 60% HFD only (n = 13-15/strain) (B). The mice were weighed weekly, oral glucose tolerance tests (OGTTs) were performed at the start of the study and at different time-points during the studies and a DEXA scan was run at the end of the studies. In the female 60% HFD study, food intake was recorded for 4 days at 3 different occasions. C; Male <i>Trpm5</i>^<i>-/-</i> and wild type mice were fed 60% HFD (n = 8/strain) from the age of 15 weeks.The mice were weighed weekly and energy expenditure, OGTT and DEXA scan were performed before and after the diet treatment. FI = Food Intake, EE = Energy Expenditure.