MnTBAP treatment alters insulin-stimulated PKB phosphorylation but not content in EWAT from mice fed a HFD.

<p>Mice were fed a LFD or HFD for 5 months and then treated with or without MnTBAP (10 mg/kg) daily for 5 weeks. At 15 min following an intraperitoneal injection of insulin (2 U/Kg), EWAT was excised and rapidly frozen in liquid nitrogen for subsequent Western blot experiments. <b>(A)</b> Representative PKB immunoblots for pThr³⁰⁸ and pSer⁴⁷³ PKB as well as total PKB-α and PKB-β isoforms. α-tubulin was used as a loading control. Quantification of <b>(B)</b> pThr³⁰⁸ PKB, <b>(C)</b> pSer³⁰⁸ PKB, <b>(D)</b> PKB-α and <b>(E)</b> PKB-β content, each normalized to α-tubulin. *, Denotes statistically significant difference from HFD-Vehicle. ⁺, Denotes statistically significant difference from LFD-MnTBAP. N = 5–6 mice per group.