Expression of T/NK module genes may be oppositely associated with patient survival.

<p>A) Overexpression of FLT3LG.mod genes is positively associated with survival of melanoma patients. Shown is a KM plot depicting survival of melanoma patients stratified by expression of FL3LG.mod. FLT3LG mod hi, tumors having greater than median expression of module genes; FLT3LG.mod lo, tumors having less than or equal to than median expression of module. B). Network graph of FLT3LG.mod genes from a protein-protein interaction network shows interconnected T cell genes. C) Overexpression of PDCD1.mod genes is positively associated with survival of head and neck tumor patients. Shown is a KM plot depicting survival of head and neck tumor patients stratified by expression of PDCD1.mod. D). Network graph of PDCD1.mod genes shows interconnected T cell genes. E) Overexpression of FOXP3.mod genes is negatively associated with survival of bladder tumor patients. Shown is a KM plot depicting survival of bladder tumor patients stratified by expression of FOXP3.mod. F) Network graph of FOXP3.mod genes shows interconnected T cell genes, including Treg genes FOXP3, IL2RA and CTLA4. G-I) Tumors partitioned by levels of T/NK cell transcripts also show differences in levels of T cell protein, LCK. LCK expression was measured by RPPA. G) LCK protein levels in FLT3LG.mod hi versus FLT3LG.mod lo melanomas. H) LCK protein levels in PDCD1.mod hi versus PDCD1.mod lo head and neck tumors. I) LCK protein levels in FOXP3.mod hi versus FOXP3.mod lo bladder tumors.