Transgene copy number determination using quantitative real-time PCR by comparison of transgenic lines with external plasmid controls.

<p>^A Average values of crossing point from three sample replicates.</p><p>^B Standard deviations.</p><p>^C Average values of extrapolated concentration relative to a single transgene copy.</p><p>^D Copy number.</p><p>^E Plasmid DNA used for copy number calculations</p><p>Transgene copy number determination using quantitative real-time PCR by comparison of transgenic lines with external plasmid controls.