Flow cytometry gating and FLOCK populations.

<p>The manual gating strategy used to gate for the CD4+ T cells is shown in the top panel (A). The CD4+ T cell events were uploaded to immPort (immport.niaid.nih.gov) for FLOCK analysis. The unique populations identified by FLOCK using the single HIV reference (bottom left) (B) and artificial reference (top right) (C) is shown. The artificial reference is made-up of 2% of the CD4+ T cells from each subject in the HIV cohort, where as the single HIV reference is made-up of the CD4+ T cells from a single individual from the HIV cohort that appeared to be biologically representative of the cohort.