SARA subcellular distribution in young cultured hippocampal pyramidal neurons.

<p>(A) Confocal image showing an example of stage 2 neurons (1DIV) or (B) stage 3 neurons (2-3DIV) immunostained for SARA (green). Note that SARA immunolabeling is present in the soma, in all types of neurites (minor process and axon) and that it extends into neuritic tips. SARA is associated with early and recycling endosomal compartments. (C-H) Neurons double-labeled, using antibodies against EEA1 (green, C) and SARA (red, D) or Rab11 (green, F) and SARA (red, G). The SARA-labeled punctae displayed partial colocalization with the early endosomal marker (E, inset) or recycling endosomal marker (H, inset). Different heterogeneous populations of endosomes were observed: one containing only EEA1 or Rab11, a second with EEA1 or RAb11 and SARA, and a third containing only SARA endosomes. All cells were imaged by confocal microscopy. Data is typical of over 10–20 neurons imaged for each condition.