Analysis of obscurin protein expression in tissue samples.
- Steven Marston (799975)
- Cecile Montgiraud (5665135)
- Alex B. Munster (799977)
- O’Neal Copeland (799978)
- Onjee Choi (799979)
- Cristobal dos Remedios (566399)
- Andrew E. Messer (799980)
- Elisabeth Ehler (86394)
- Ralph Knöll (799981)
Publication date
September 2015
DOIAbstract
<p>Left: Identification of obscurin in cardiac myofibrils by 2% agarose/2% PAA/SDS gel electrophoresis. Left, SYPRO Ruby protein stain, Right Western blot Ob59 antibody. Centre: Obscurin expression in myofibrils. Western blot of 4–18% gradient SDS-PAGE. Left: membrane stained with MemCode protein stain, right: probed with Ob59 antibody. For quantification obscurin was normalised to α-actinin. Right: Identification of obscurin in whole heart muscle homogenates. Top, MemCode protein stain; bottom, Western blot with Ob59 antibody.</p
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