ADAM12-dependent EMT does not require catalytic activity and is specific of ADAM12L.

<p>MCF10A cells were infected with lentiviruses expressing either the fusion proteins GFP-ADAM12L, GFP-ADAM12-E351Q, GFP-ADAM12S or the control protein GFP (Control). After antibiotic selection, cells were further enriched using flow cytometry and cultured for 48 hours. (A) Bright field pictures were captured to show cell morphology. Cells were fixed and immunostained for E-cadherin or stained with rhodamine-conjugated phalloidin to monitor actin stress fibers cytoskeletal actin. (B) The levels of vimentin and E-cadherin were determined by western blot analyses. Left, representative western blots. Immunoblots for ADAM12L, ADAM12S and HSC-70 are shown as control. Right, densitometric analysis of protein amounts. Results are expressed as mean±SD from 6 independent experiments (*, p<0.05; **, p<0.01).