Stimulation of DNA ligation by histone H1 and deletion mutants.

<p>The 5´-end ³²P-labeled 123-bp DNA fragment (~1 nM) was pre-incubated with 1–15 nM (<i>left</i> to <i>right</i>) histone H1 (fl) or deletion mutants within the highly basic C-terminus, followed by ligation by T4 DNA ligase. Deproteinised DNA samples were separated by electrophoresis on 5% non-denaturing polyacrylamide gels in 0.5x TBE buffer.