C-STrap processing of 50 μg of protein from HeLa lysates from each of 3 replicates, using either the basic (A) or only quartz (OQ) (B) approaches, followed by the LC-MS/MS analysis.

<p>On average per sample we identified 4280 proteins with the basic approach and 4930 proteins with the OQ approach. The occurrence of the cysteinyl peptides in the Cys fractions was 82.3% and 91.8% (average per sample) using the basic and OQ approaches, respectively.