Dose-dependent neutralization of genotypes/subtypes 1–6 HCVpp with mAbs 2O18 and 2C21.

<p>2O18 and 2C21 were 2-fold diluted, from 128 μg/ml to 1 μg/ml, mixed with HCVpp and incubated for 1 hour and then added to Huh7.5 cells. Seventy-two hours post-infection, viral infection was evaluated by determining luciferase activity. HCVpp without antibody served as the positive control. One-half inhibitory concentrations for virus neutralization values (IC₅₀) against each HCVpp were calculated using GraphPad Prism software. NA, not applicable. The experiment was performed in triplicate and the error bars represented the standard error of the means (SEM).