CIO effect on wound healing.

<p>Cell migration ability was determined by scratching a confluent HaCaT cell monolayers with a pipette tip. Cells were incubated with olive oil or various concentrations of CIO (diluted in olive oil) for 15 min followed by a 24h-recovery period. Olive oil (as negative control) compared to PBS (as cell control) didn’t exert an inhibitory effect on wound closure (data not shown). Microscopic bright field pictures were taken after the scratch and the precise coordinates were reassessed 24h after incubation. (<b>A</b>) Example of scratch assay in olive oil; (<b>B-F</b>) Examples of scratch assays at 0.1% CIO1-5; (<b>B’-F’</b>) CIO1-5 quantification of wound closure at 0.01, 0.1 and 1% concentration. CIO1-5 were compared to olive oil as control using t-tests. n-s: non-significant, *: 0.01 < p < 0.05, **: 0.01 < p, ***: 0.001 < p.