<p>(A) The strains were spotted onto VFMG-CF agar plates supplemented with or without 0.02% of arabinose and 10 mM of CaCl<sub>2</sub>. Then, the plates were incubated at 30°C for 3 d. Ampicillin and kanamycin (200 μg/ml for each) were used to maintain plasmids in the strains. Each colony representing the mean rugosity of colony morphology from at least three independent experiments was visualized using a stereomicroscope (Stemi DV4, Zeiss) at an 8× magnification. Bars, 1 mm; JN111 (pJK1113), parent strain; <i>cabA</i> (pJK1113), <i>cabA</i> mutant; <i>cabA</i> (pYM1109), complemented strain.</p
<p>(<b>A</b>). Schematic representation of the screening pipeline. Cells seeded in 96-well plates in...
<p>(A) Colony morphology of the wild type (PH-1), <i>FgATG9</i> deletion mutants (Δ<i>Fgatg9-2</i>, ...
<p>WT: the wild-type strain; KO: <i>Pks1</i> knockout mutants; KD: <i>Pks1</i> knockdown mutants. Pi...
<p>(A and C) Biofilms of the strains were grown for 24 h on 96-well microtiter plate wells containin...
<p>(A) Biofilms of the strains were grown with VFMG-CF supplemented with 0.01% arabinose, and variou...
<p>Biofilms of the strains were grown for 3 d in the flow cells containing VFMG-CF supplemented with...
<p>For each strain a 10 µl aliquot of glycerol stock was spread across a 1% (w/v) HIS agar plate. Af...
<p>(A) Biofilms of the co-cultures prepared by mixing JN111, the <i>cabA</i> mutant, or the <i>brpA<...
<p>Representative colonies of wild type PG2, mutant (Δ<i>pdhB</i>) and complemented (Δ<i>pdhB</i>:: ...
<p>The colony morphology was observed by an optical microscope after culturing. The J15cs strain for...
<p>The normal chiral morphology of B. mycoides when grown from a single central point of inoculation...
<p>For each strain a 15 µl spot of overnight culture was placed onto a 1% (w/v) HIS agar plate which...
<p>(A) Invasive growth assays for strains of the indicated genotypes on 2% agar. Cells were spotted ...
<p>(<b>A</b>) SC5314 wild-type non-filamentous growth on YPD agar (30° C); (<b>B</b>) SC5314 wild-ty...
<p>(<b>A</b>) Shown are representative images of LB agar plates of AB1157 transformants following 18...
<p>(<b>A</b>). Schematic representation of the screening pipeline. Cells seeded in 96-well plates in...
<p>(A) Colony morphology of the wild type (PH-1), <i>FgATG9</i> deletion mutants (Δ<i>Fgatg9-2</i>, ...
<p>WT: the wild-type strain; KO: <i>Pks1</i> knockout mutants; KD: <i>Pks1</i> knockdown mutants. Pi...
<p>(A and C) Biofilms of the strains were grown for 24 h on 96-well microtiter plate wells containin...
<p>(A) Biofilms of the strains were grown with VFMG-CF supplemented with 0.01% arabinose, and variou...
<p>Biofilms of the strains were grown for 3 d in the flow cells containing VFMG-CF supplemented with...
<p>For each strain a 10 µl aliquot of glycerol stock was spread across a 1% (w/v) HIS agar plate. Af...
<p>(A) Biofilms of the co-cultures prepared by mixing JN111, the <i>cabA</i> mutant, or the <i>brpA<...
<p>Representative colonies of wild type PG2, mutant (Δ<i>pdhB</i>) and complemented (Δ<i>pdhB</i>:: ...
<p>The colony morphology was observed by an optical microscope after culturing. The J15cs strain for...
<p>The normal chiral morphology of B. mycoides when grown from a single central point of inoculation...
<p>For each strain a 15 µl spot of overnight culture was placed onto a 1% (w/v) HIS agar plate which...
<p>(A) Invasive growth assays for strains of the indicated genotypes on 2% agar. Cells were spotted ...
<p>(<b>A</b>) SC5314 wild-type non-filamentous growth on YPD agar (30° C); (<b>B</b>) SC5314 wild-ty...
<p>(<b>A</b>) Shown are representative images of LB agar plates of AB1157 transformants following 18...
<p>(<b>A</b>). Schematic representation of the screening pipeline. Cells seeded in 96-well plates in...
<p>(A) Colony morphology of the wild type (PH-1), <i>FgATG9</i> deletion mutants (Δ<i>Fgatg9-2</i>, ...
<p>WT: the wild-type strain; KO: <i>Pks1</i> knockout mutants; KD: <i>Pks1</i> knockdown mutants. Pi...