Additional file 2: Figure S1. of Diversity in boron toxicity tolerance of Australian barley (Hordeum vulgare L.) genotypes

Nine barleys accessioned with the name Sahara contain the rare HvBot1 gene duplication. (a) Southern analysis of allele type and gene duplication at HvBot1, for a representative set of barley genotypes. Lane 1, Clipper; 2, Sahara 3771; 3; Alexis; 4, Amagi Nijo; 5, Arapiles; 6, Atlas; 7, Barque; 8, California Mariout; 9, Chebec; 10, CM72; 11, Commander; 12, Flagship; 13, Fleet; 14, Franklin; 15, Gairdner; 16, Galleon; 17, Golden Promise; 18, Halcyon; 19, Haruna Nijo; 20, Clipper; 21, Sahara 3771; 22, Keel; 23, Morex; 24, Mundah; 25, Sahara 3763; 26, Sahara 3764; 27, Sahara 3765; 28, Sahara 3766; 29, Sahara 3767; 30, Sahara 3768; 31, Sahara 3769; 32, Sahara 3770, 33, Schooner; 34, Skiff; 35, Sloop; 36, Steptoe; 37, Tadmor; 38, Tokak. Genomic DNA was digested with Dra I, and probed with a [32P]-labelled nucleic acid fragment of HvBot1 derived from Clipper (Additional File 5: Table S3). (b) Leaf symptom scores (upper panel) and shoot B concentrations (lower panel) of nine Sahara barley accessions grown in nutrient solutions with 5Â mM additional B (PDF 1165 kb