Colonoid gene expression changes induced by Poly I:C stimulation.

<p>Colonoids were grown and stimulated as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0138531#pone.0138531.g002" target="_blank">Fig 2</a>. Gene expression levels were determined using Nanostring probe-based analysis of isolated RNA. <b>A)</b> Fold change in normalized gene expression are grouped by category. Bars represent mean ±SEM compared to unstimulated samples. n = 6 (Poly I:C stimulation)/5 (unstim) duplicate samples. Discoveries (*) determined by false discovery rate following multiple t-test analysis. <b>B)</b> Heat map of the normalized probe counts of unstimulated and Poly I:C stimulated colonoids of each technical replicate. Samples and genes were clustered by Pearson correlation.