Growth morphology and survival is impacted by Poly I:C stimulation of enteroids.

<p>Crypts from the jejunum were plated in Matrigel for two days. Pam3CSK4 (400ng/mL), Poly I:C (40μg/mL) or LPS (13ug/mL) was added to the media on day 2 and day 4. Two days after the last addition of Poly I:C, (D6) images were obtained from at least four areas of interest of triplicate wells at multiple depths of focus. <b>A)</b> Buds on individual enteroids were counted using images at multiple depths of focus, n = 6 individual jejunal preparations. <b>B)</b> The surface area of the enteroids was measured using the free hand drawing tool in the NIS-Elements software, n = 6. <b>C)</b> The percentage of enteroids surviving stimulation to day 6 compared to unstimulated controls was calculated in triplicate wells, n = 3. <b>D)</b> Images of unstimulated and stimulated enteroids. Images were taken at 4X, scale bars represent 100μm. Bars represent mean ± SEM. Significance determined by One-way ANOVA, Dunnett’s multiple comparison test, * p<0.05, **** p<0.0001.