PGDM inhibited cell proliferation in prenatal mouse hearts.

<p>PH3 immunofluorescent staining (red) was performed on the vertical sections of the prenatal mouse hearts. All of the sections were counterstained with DAPI (blue). <b>A1-A3/B1-B3</b>: The vertical sections of E18.5 mouse hearts from the control cardiac VS (A1), RVW (A2), and LVW (A3) and PGDM cardiac VS (B1), RVW (B2), and LVW (B3). <b>C1-C3/D1-D3</b>: The vertical sections of E15.5 mouse hearts from the control cardiac VS (C1), RVW (C2), and LVW (C3) and PGDM cardiac VS (D1), RVW (D2), and LVW (D3). <b>E1-E3/F1-F3</b>: The vertical sections of E13.5 mouse hearts from the control cardiac VS (E1), RVW (E2), and LVW (E3) and PGDM cardiac VS (F1), RVW (F2), and LVW (F3). <b>G</b>: The bar charts showing the numbers of PH3⁺ cells in the VS, RVW and LVW of the E18.5 control and PGDM mice. <b>H</b>: The bar charts showing the numbers of PH3⁺ cells in the VS, RVW and LVW of the E15.5 control and PGDM mice. <b>I</b>: The bar charts showing the numbers of PH3⁺ cells in the VS, RVW and LVW of the E13.5 control and PGDM mice. **<i>P</i><0.01, ***<i>P</i><0.001 vs control. Scale bars: 100 μm in A1-F3.