Nkx2.5 expression was suppressed in E18.5 and E15.5 PGDM mice.

<p>Nkx2.5 immunofluorescent staining (red) was performed on the vertical sections of the prenatal mouse hearts. All of the sections were counterstained with DAPI (blue). <b>A-D:</b> The representative images of H&E-stained vertical sections from the E18.5 control (A), E18.5 PGDM (B), E15.5 control (C) and E15.5 PDGM (D) mice. <b>A1-A3/B1-B3</b>: The vertical sections of the E18.5 mouse hearts from control cardiac VS (A1), RVW (A2), and LVW (A3) and PGDM cardiac VS (B1), RVW (B2), and LVW (B3). <b>C1-C3/D1-D3</b>: The vertical sections of E15.5 mouse hearts from control cardiac VS (C1), RVW (C2), and LVW (C3) and PGDM cardiac VS (D1), RVW (D2), and LVW (D3). <b>E</b>: Bar charts showing the percentage of Nkx2.5⁺ cells/DAPI cells in the VS, RVW and LVW of the E18.5 control and PGDM mice. <b>F</b>: Bar charts showing the percentage of Nkx2.5⁺ cells/DAPI cells in the VS, RVW and LVW of the E15.5 control and PGDM mice. <b>G</b>: Western blotting data showing Nkx2.5 expression at the protein level in E18.5 or E15.5 control and PGDM mice. *<i>P</i><0.05, ***<i>P</i><0.001 vs control. Scale bars: 400 μm in A-D and 100 μm in A1-D3.