The change of the phenotypes of GFP+ cells within tumors.

<p>(A) The percentage of GFP+ cells co-express CD45, CD11b, F4/80, or CD68 antigen as assayed by flow cytometry for the monocytes differentiated from the bone marrow cells harvested from C57BL/6-Tg(CAG-EGFP)1Osb/J mice <i>in vitro</i>. Bar: SE of 3 independent experiments. (B) The percentage of GFP+ cells co-express CD45, CD11b, F4/80, or CD68 antigen examined by immunohistochemistry (IHC) within the tumors taken from one day or one week after 25 Gy of irradiation (IR) or shame irradiated (Control). The average percentage of each surface marker of GFP+ cells of 5 randomly selected fields of each tumor from 3 tumor tissues was counted under 40X objective len. Bar: SE of 3 tumor samples. ***: P <0.005 by one way ANOVA test.