Origin of the <i>Astn2</i> exon 5 deletion.

<p>(A) PCR analysis of genomic DNA in the neighborhood of <i>Astn2</i> exon 5, as shown in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005532#pgen.1005532.g002" target="_blank">Fig 2E</a>. The ~30 kb deletion is present in 129X1/SvJ mice and R1 ES cells, but is absent from C57Bl6/J, 129S1/SvlmJ, and 129S6/SvEvTac mice. (B) The <i>Astn2</i> locus from the three 129 mouse lines shown in (A) was bred to homozygosity in a <i>Fz6</i>^<i>-/-</i> background. The <i>ridge</i> phenotype was observed only in the presence of the 129X1/SvJ <i>Astn2</i> locus; mice were photographed at P14.