Blocking of TNF receptor signaling did not affect IL-17A/polyI:C-induced proinflammatory cytokine gene expression.

<p>BEAS-2B cells were transfected with TNFR1 siRNA (solid bars) or a random oligomer negative control (shadowed bars) for 24 hours. At 72 hours after transfection, transfected cells were harvested for real-time quantitative PCR analysis following 24 hours of stimulation with IL-17A and/or polyI:C. Treatment with TNFR1 siRNAs reduced endogenous TNFR1 mRNA level about 80% as compared with the negative control (A). Expression of G-CSF and IL-8 mRNAs that were induced by polyI:C or co-treatment with IL-17A/polyI:C was not attenuated by knockdown of TNFR1 (B, C). * p < 0.001, TNFR1 siRNA vs. negative control.