Membrane permeability changes of BGC-823 cells by monitoring PI and LDH.

<p>The cells were incubated with increasing peptide concentrations for 1 h at 37 oC. (A) Quantitative comparisons of fluorescence intensity (in Geomean) at various concentrations were analyzed by flow cytometry. (B) LDH in the supernatant was measured with a microplate reader at 450 nm. Cells without treatment or lysed with triton X-100 was used as negative and positive controls, respectively. LDH activity was calculated as the percentage of experimental group and positive control, after subtraction of negative control respectively (*P<0.005). Data are the mean ± SD of three independent experiments.