PNG+aFGF+ChABC treatment induces 5-HT and TH regeneration 18 weeks post-complete spinal cord transection.

<p>TX-Only: (A) Camera lucida tracing of 5-HT immunoreactive fibers in a TX-only animal. (B) 5-HT and GFAP immunostaining of the boxed area in (A), scale bar, 50 μm. (C) Camera lucida tracing of TH-immunoreactive fibers in a TX-only animal. (D) TH and GFAP immunostaining of the boxed area in (C). Scale bar, 50 μm. PNG+aFGF+ChABC: (E) Camera lucida tracing of 5-HT-immunoreactive fibers in PNG+aFGF+ChABC. (F) 5-HT and GFAP immunostaining of boxed areas in (E): (F’) Rostral spinal cord-PNG interface; (F”) within the PNG; (F”‘) caudal spinal cord. Scale bar, 50 μm. (G) Camera lucida tracing of TH-immunoreactive fibers in a PNG+aFGF+ChABC-treated animal. (H) TH and GFAP immunostaining of boxed areas in (G): (H’) Rostral spinal cord-PNG interface; (H”) within the PNG; (H”‘) caudal spinal cord. Scale bar, 50 μm. (I) Quantification of 5-HT-immunoreactive fibers found in caudal spinal cord, n = 7. (J) Quantification of TH-immunoreactive fibers found in caudal spinal cord, n = 7. Only animals whose spinal cord was processed and cut sagittally were included in the analysis.