Additional file 3: Figure S3. of Transformation of enriched mammary cell populations with polyomavirus middle T antigen influences tumor subtype and metastatic potential

PyMT expression and activity in tumors. a RT-qPCR quantification of relative PyMT mRNA expression levels in individual MMTV-PyMT, luminal CD133+ cell, luminal CD133− cell, basal cell, and stem cell tumors normalized to the Rplp0 housekeeping gene. Analysis was performed in triplicate for each tumor sample. b Western blot quantification of relative ratio of pAKT to AKT protein expression in individual MMTV-PyMT, luminal CD133+ cell, luminal CD133− cell, basal cell, and stem cell tumors normalized to β-actin. c Western blot quantification of relative ratio of pERK to ERK protein expression in individual MMTV-PyMT, luminal CD133+ cell, luminal CD133− cell, basal cell, and stem cell tumors normalized to β-actin. d Western blot quantification of relative ratio of pSRC to SRC protein expression in individual MMTV-PyMT, luminal CD133+ cell, luminal CD133− cell, basal cell, and stem cell tumors normalized to β-actin. (TIFF 2407 kb