Additional file 2: Figure S2. of Transformation of enriched mammary cell populations with polyomavirus middle T antigen influences tumor subtype and metastatic potential

Hormone independence of EF1α-PyMT-ZsGreen-transduced MEC tumors. a Half-maximal inhibitory concentration values of wild-type MECs and cells isolated from luminal CD133+, luminal CD133−, basal, and stem cell tumors treated for 48 h with 4-hydroxytamoxifen (4-OHT). No significant differences among the viability of tumor cells and normal MECs were observed after 4-OHT treatment (t test). b Assessment of tumor growth response to ovariectomy. Transduced primary cells were transplanted into ovariectomized mice to test if lack of hormone signaling would result in reduced tumor growth from luminal CD133+ cells. No differences in tumor latencies were observed among tumor groups (n = number of mice). (TIFF 1112 kb