Flt3L-dependent migratory DCs and monocyte-derived DCs both present <i>C</i>. <i>albicans</i> derived antigen.

<p><b>(A)</b> Cervical lymph node cells of B6 or <i>Flt3l</i>-/- mice were analyzed on day 2 post-infection. Representative FACS plots from individual mice and quantification of MHC II^high CD11c⁺ (population I), MHC II⁺ CD11c^high (population II) and MHC II⁺ CD11c⁺ cells (population III) from one of two independent experiments are shown. <b>(B, C)</b> Cervical lymph node cells were isolated from naïve B6 mice and from infected B6 and <i>Flt3l</i>-/- mice on day 2 post-infection, enriched for CD11b⁺ cells and co-cultured with CD4⁺ Hector T cells. Thy1.1⁺ CD3⁺ CD4⁺ TCRVα2⁺ cells were analyzed for CD69 expression after 1 day (B) and for proliferation after 4 days, respectively (C). Representative plots are shown on the left, summary of data from individual mice with mean + SD are shown on the right. Each symbol represents one mouse. Data are representative of 2 independent experiments. (<b>D</b>) As in B, but MHC II^high CD11c⁺ migratory DCs were isolated by FACS-sorting from the cervical lymph nodes of infected B6 and <i>Flt3l</i>-/- mice. (<b>E—F</b>) CCR2⁺ CD11b⁺ cells in the cervical lymph nodes of naïve and infected B6 mice on day 2 post-infection were analyzed for the expression of MHC II and CD11c (D). Three distinct subsets of CCR2⁺ CD11b⁺ cells (MHC II^lo/int Ly6C⁺ (Q1), MHC II^int Ly6C^- (Q2) and MHC II^high Ly6C^- (Q3)) from infected mice were further analyzed for the expression of CCR2 and CD11c (E). Representative FACS plots are shown. <b>(G)</b> Cervical lymph nodes were isolated from infected B6 mice on day 2 post-infection. CCR2^hi, CCR2^int and CCR2^lo subsets within the MHC II^high CD11c⁺ population I were FACS-sorted and co-cultured with CD4⁺ Hector T cells for 1 day. Thy1.1⁺ CD3⁺ CD4⁺ TCRVα2⁺ cells were then analyzed for CD69 expression. Representative FACS plots are shown, Data are representative of 2 independent experiments.