Antigen-specific Th cell response to <i>C</i>. <i>albicans</i> oropharyngeal infection.

<p>(<b>A, B</b>) CFSE-labelled CD4⁺ Hector T cells were adoptively transferred one day prior to sublingual infection of B6 mice with <i>C</i>. <i>albicans</i>. Proliferation of Thy1.1⁺ CD4⁺ Vα2⁺ Hector cells was analyzed by flow cytometry on day 3 post-infection. A representative FACS plot is shown in (A), quantification of Thy1.1⁺ CD4⁺ Vα2⁺ Hector cells in cervical lymph nodes is shown in (B). Each symbol represents an individual mouse, the mean + SD is indicated, data are pooled form 2 independent experiments. (<b>C—E</b>) CD4⁺ Hector T cells were adoptively transferred one day prior to sublingual infection with <i>C</i>. <i>albicans</i>. In (C), Thy1.1⁺ CD4⁺ Vα2⁺ Hector cells in the cervical lymph nodes were analyzed on day 7 post-infection for expression of CD44 and CD62L. Representative FACS plots from 3 independent experiments are shown. In (D—E), cytokine production by endogenous CD3⁺ CD4⁺ Thy1.2⁺ T cells (left) and Thy1.1⁺ CD4⁺ Vα2⁺ Hector cells (right) on day 7 post-infection was analyzed by flow cytometry after re-stimulation with DC¹⁹⁴⁰ cells pulsed with heat-killed (h.k.) <i>C</i>. <i>albicans</i> or pADH1_126-140 as indicated. Representative FACS plots are shown in C and D, quantification of IL-17-producing cells is shown in (E). Each symbol represents an individual mouse, the mean + SD is indicated, data are pooled form 2 independent experiments. (<b>F, G</b>) B6 mice obtained an adoptive transfer of CD4⁺ Hector T cells one day prior to systemic infection with 5 x 10⁴ cfu <i>C</i>. <i>albicans</i>. Cytokine production by endogenous CD3⁺ CD4⁺ Thy1.2⁺ T cells (left) and Thy1.1⁺ CD4⁺ Vα2⁺ Hector cells (right) in the renal lymph nodes (left) and spleen (right) on day 7 post-infection was analyzed after re-stimulation with DC¹⁹⁴⁰ cells pulsed with heat-killed (h.k.) <i>C</i>. <i>albicans</i> or pADH1_126-140 as indicated. Representative FACS plots are shown in (F), quantification of IFN-γ-producing cells is shown in (G). Each symbol represents an individual mouse, the mean + SD is indicated, data are pooled form 2 independent experiments.