<i>as2</i> mutant is resistant to CaLCuV infection.

<p>(A) WT (Col-0), <i>as1-1</i> or <i>as2-1</i> plants infected with CaLCuV. Note typical viral symptoms of early-stage flowers (10 dpi) in WT and <i>as1-1</i> inflorescence. Bar = 10mm. (B) WT and mutant plants infected with CaLCuV. Pictures were taken at 28 dpi. Bar = 10mm. (C) Flowers of WT and mutant plants from (B) (28 dpi). Bar = 10mm. (D) Relative CaLCuV DNA levels (DNA-A and DNA-B) in infected (by particle bombardment) WT, <i>as1-1</i>, and <i>as2-1</i> plants (28 dpi). <i>Actin2</i> levels served as an internal plant genomic DNA control. Values are mean ± SD (n = 3 biological replicates). (E) Relative CaLCuV DNA levels (DNA-A and DNA-B) in infected (by particle bombardment) WT, <i>as2-1</i>, and <i>35S</i>:<i>AS2</i> overexpression (<i>35S</i>:<i>AS2</i>) plants (18 dpi). <i>AtActin2</i> levels served as an internal plant genomic DNA control. Values are mean ± SD (n = 3 biological replicates).