Design and assembly of the microfluidic culture system.

<p>a: Schematic section of a culture well: cells are cultured on a Transwell^® inserted in the holder. A V-ring is used to seal the system. b: Schematic of the setup: each well is connected to the syringe pump. A plate is placed under the device and is moved to allow for the time-resolved fraction collection. c: Exploded view of the assembly of a single well. Five layers of dry film photoresist are laminated on top of a support layer. A holder is screwed on top of the support layer and a Transwell^® is subsequently inserted together with the V-ring. The microfluidic inlet is screwed in the holder while the outlet needle is glued on the bottom of the support layer.