Comparison of antigen immobilization methods.
- Nan Zhong (803476)
- Peter Loppnau (273605)
- Alma Seitova (499895)
- Mani Ravichandran (326517)
- Maria Fenner (803477)
- Harshika Jain (803478)
- Anandi Bhattacharya (108629)
- Ashley Hutchinson (803479)
- Marcin Paduch (803480)
- Vincent Lu (246204)
- Michal Olszewski (803481)
- Anthony A. Kossiakoff (237521)
- Evan Dowdell (803482)
- Akiko Koide (141529)
- Shohei Koide (141562)
- Haiming Huang (803483)
- Vincent Nadeem (803484)
- Sachdev S. Sidhu (141559)
- Jack F. Greenblatt (196560)
- Edyta Marcon (803485)
- Cheryl H. Arrowsmith (246847)
- Aled M. Edwards (463724)
- Susanne Gräslund (161738)
Publication date
October 2015
DOIAbstract
<p>(A) Three different affinity tags were tested for antigen immobilization in phage display; <i>in vivo</i> biotinylation through an Avi tag, SBP and GST tags. The diversity of Fabs derived from these differentially tagged antigens was then compared. (B) Immunoprecipitation with Fabs selected against either Avi-tagged antigen or GST-tagged antigen from a cell lysate expressing FLAG-tagged target protein. Immunoprecipitated antigen was detected with an M2 antibody against the FLAG tag. Fabs selected against Avi-tagged antigen generally show a higher recovery of the antigen.</p
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