AG–4 induces anti-proliferative effect.

<p>(A) Time-dependent effect in U937 cells. U937 cells were treated with AG–4 (0–50 μM) for 24, 48 or 72 hours. Cell death was assessed by MTS-PMS assay. Each data point represents the mean±SEM of at least three independent experiments in duplicate. (B) Effect on other cells. Human cancer cell lines (Raji, MCF–7, HCT–15) and healthy human PBMC were incubated with AG–4 (0–50 μM) for 48 h. Cell death was assessed by MTS-PMS assay. Each data point represents the mean±SEM of at least three independent experiments in duplicate.