Cardiac CTGF expression induced by a high SRF overexpression.

<p>(A) Western blot analysis of CTGF protein. The GAPDH blot represented here is the same used for vimentin in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0139858#pone.0139858.g004" target="_blank">Fig 4</a> since CTGF and vimentin were blot on the same membrane. (B) Quantification of CTGF protein level (n = 4). Data are presented as means ± s.e.m. * and *** indicate significant difference at P < 0.05 and P < 0.001, respectively versus the control group. (C) Immunoflurescence labeling of SRF (red), vinculin (green) and nuclei (blue) (upper line), and CTGF (orange), vinculin (green) and nuclei (blue) (lower line) on serial heart sections of control, SRF-LL and SRF-HL mice. The white arrows indicate SRF-positive/CTGF-null cardiomyocytes while the orange arrows indicate SRF-positive/CTGF-positive cardiomyocytes. All these data presented in this figure are representative of three independent experiments. Scale bar: 80 μm.