IFN-γ secretion is disturbed in CD98hc^f/f-CD4 mice.

<p>(A) CFSE labeled spleen cells from CD98hc^f/f-CD4-OT11 or CD98hc^+/+-CD4-OT11 mice were stimulated with OVA peptides under Th1 culture conditions for 3 days. Cells were stained with anti-CD4, anti-CD98hc and anti-IFN-γ mAbs and evaluated by flow cytometry. Data shown are gated on CD98hc^- cells. (B) Spleen cells from CD98hc^f/f-CD4-OT11 or CD98hc^+/+-CD4-OT11 mice were stimulated with OVA peptides under Th1 culture conditions for 3 days. Cells were stained with anti-CD4 and AnnexinV, and evaluated by flow cytometry. (C) Cells from CD98hc^f/f-CD4-OT11 (Thy1.2) or CD98hc^+/+-CD4-OT11 (Thy1.1⁺/Thy1.2⁺) mice were labeled with CFSE and transferred into CD45.1 C57BL/6 mice. Mice were then immunized with OVA protein. CFSE dilution and intracellular INF-γ expression were evaluated by flow cytometry gated on CD98hc⁺ (CD98hc^+/+-CD4-OT11) or CD98^- cells (CD98hc^f/f-CD4-OT11) cells four days after immunization (left). The percentages of INF-γ⁺ cells among total CD4⁺ T cells are shown (middle). The percentages of INF-γ⁺ cells among total CD4⁺ cells that underwent cell division at the indicated times were counted (right). Results are means ± S.D. of 4 mice; * significant difference (<i>p</i><0.05). Data shown in this Figure are representative of three experiments.