Inflammation induces S-nitrosylation in zebrafish larvae.

<p>Changes in total protein S-nitrosylation during inflammation was assessed with the modified biotin switch technique and detected by Western Blot. S-nitrosylated proteins in samples of control (lane 1) larvae and larvae with chemically induced inflammation (lane 2 and 3) were detected by anti-TMT immunoblotting. Chemically induced inflammation significantly induces S-nitrosylation, marked by additional strong anti-TMT immunoblotting signals (arrowheads), in protein extracts of CuSO₄ treated larvae. Inhibition of Nos with 250 μM L-NAME prior to CuSO₄ treatment prevents changes in protein S-nitrosylation. EF–1α was used as loading control.