PLCβ4 is critical for the inhibition of LPL expression in visceral adipocytes.

<p>(A) siRNA specific to PLCβ4 reduced PLCβ4 expression in visceral adipocytes. Visceral adipocytes were transfected with PLCβ4-siRNA or scrambled siRNA, and incubated for 12 h to measure the enzyme expression levels by western blot analysis. (B, C) AngII reduced LPL expression via PLCβ4 in visceral adipocytes. After transfection with PLCβ4-siRNA or of scrambled siRNA, adipocytes were incubated with AngII (1 μM), PMA (10 or 100 nM), or vehicle (untreated). After 12 h incubation total protein (B) and total RNA (C) was extracted for measurement of LPL protein and mRNA levels, respectively. Each column and bar represents the mean ± SEM for three separate experiments. An asterisk (*) indicates <i>p</i><0.05 vs. control. In panel A and B, the ratio of PLCβ4 or LPL protein to β-actin was calculated based on densitometric quantification of the bands. In panel C, LPL mRNA levels were normalized to β-actin.