Representative mass spectrometry data from quantitative analysis of HSPA1-Lys561 methylation status.

<p>(A)-(D) Methylation status of Lys561 in HSPA1 in tumor sample # 14. Left panels, chromatograms were generated by gating for mass-to-charge ratios of the (A) unmethylated (me0), (B) monomethylated (me1), (C) dimethylated (me2) and (D) trimethylated (me3) state of the AspN-generated proteolytic peptide encompassing Asp555-Ala565 in HSPA1. The elution time (arrow) and the area under each curve (in brackets), as well as the calculated relative abundance (as percentage) of the various lysine methylation states, are indicated. Right panels, annotated tandem mass spectra supporting the identity of analyzed peptides.