Characterization of renal ischemia reperfusion (I/R) model in C57BL/6J wild type mice.

<p>(A) Serum urea levels were measured with the Analisa kit in Sham mice (first column) and the I/R group at days 5, 8 and 15 after injury. (B) Vimentin mRNA levels in renal tissue (black bars, right kidney; gray bars, left kidney) were measured in the Sham (first column) and I/R groups at days 5, 8, 12 and 15 after injury. (C) Left kidney weight /body weight ratio (LKW/BW) measured in Sham mice and animals killed at the times after I/R indicated on the abscissa. (D) RKW/BW: the same for right kidney. (E) Proton density magnetic resonance renal images. I: axial plane view in a Sham mouse 15 days after surgical manipulation; II and III: axial planes of proton density magnetic resonance images from 2 different I/R mice 15 days after reperfusion; IV: coronal view of the abnormal kidney shown in III. (F to H) Serum levels of TNF-α, IFN-γ and IL-1β measured in the Sham group (0 day) and in mice after I/R at the days indicated on the abscissa. Bars data are mean ± SD. * <i>P</i> < 0.05 with respect to the corresponding Sham group (one way ANOVA followed by Bonferroni post-test for selected pairs; number of animals within the bars, where applied).