Close-up view of images of 2D-GE extracted from Fig 2.

<p>(A) Plots of phosphorylation levels of ProQ diamond stained 14-3-3 proteins. The Pro-Q diamond stained gel images in Fig 3B (a-d) are close-up views of areas of spots in WT1, <i>kl/kl</i>, WT2 and EFmKL48 full gels respectively within pI 4.3–4.8. Plots are pooled data from two replicate gels and deviations are shown. Representative ProQ diamond gel images and their corresponding positions in SyproRuby-stained protein gels are shown together to normalize for levels of protein load. Digitized values of stained spots used for the plots are shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0139914#pone.0139914.s003" target="_blank">S3 Table</a>.