MTDs cause cell death <i>in vitro</i> in a dose dependent manner.

<p>(A) MTDs (Mitochondrial Damage-Associated Molecular Patterns) induce significant rat hepatocytes cell death at concentrations above 100 ug/ml, McA RH7777 co-cultured with different concentrations of MTDs (ug/mL) for 24 hours. (B) MTDs induce significant human hepatocytes cell death at concentrations above 100 ug/ml, HepG2 co-cultured with different concentrations of MTDs for 24 hours (ug/ml). The blank bar indicates Annexin V positive cells, the black bar indicates PI positive cells. *, P = 0.01, **, P = 0.001, ***, P = 0.0001 (n = 3/group).